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Group A Streptococcus (GAS) is a very important pathogen, especially for children.On a global scale, GAS is an important cause of morbidity and mortality.But the burden of disease caused by GAS is still unknown in China and also has not obtained enough attention.For this purpose, the expert consensus is comprehensively described in diagnosis, treatment and prevention of GAS diseases in children, covering related aspects of pneumology, infectiology, immunology, microbiology, cardiology, nephrology, critical care medicine and preventive medicine.Accordingly, the consensus document was intended to improve management strategies of GAS disease in Chinese children.
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Acute respiratory tract infection is the most common infectious disease in children, which seriously threatens children′s health.Rapid and accurate etiological diagnosis is of great significance for the clinical treatment and control of these diseases.Pathogen nucleic acid test was applied and became the main method of respiratory tract infection diagnosis for its high sensitivity and specificity.To regulate the application of pathogen nucleic acid amplification test in respiratory tract infection in children, improve the diagnosis level, expert consensus on nucleic acid amplification test of respiratory pathogens in children was prepared to guide the application and promote pathogens diagnosis ability.
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Objective:To establish a common method for detecting serotypes of respiratory adenovirus, and to detect the main serotypes of respiratory human adenovirus (HAdV) infection in children in Wenzhou area.Methods:A multiplex PCR method based on capillary electrophoresis was developed to detect 12 common serotypes of respiratory adenovirus.A total of 1 059 children with acute respiratory infection who were admitted to Yuying Children′s Hospital Affiliated to Wenzhou Medical University from January 2018 to December 2019 with positive infection of HAdV detected by the direct immunofluorescence method were recruited and retrospectively analyzed.Multiplex PCR was performed to determine 12 serotypes of respiratory adenovirus, including HAdV-1, 2, 3, 4, 5, 7, 14, 21, 37, 40, 41 and 55.Meanwhile, some samples were randomly selected to examine the consistency in the detection result by the first-generation sequencing method.Results:A total of 1 059 specimens of respiratory secretions with positive HAdV antigen were collected.Detected by multiplex PCR method, 947 cases (89.4%) were positive for 1 serotype, 13 cases (1.2%) were mixed infection with 2 serotypes, and 24 cases (2.3%) were negative.In addition, 75 cases(7.1%) were positive but could not be serotyped.Among the 947 children with the positive infection of a single serotype, 415 cases (43.8%) were HAdV-3 in subgroup B, 318 cases(33.6%) were HAdV-7, 12 cases (1.2%) were HAdV-55, 2 cases (0.2%) were HAdV-21, 108 cases (11.4%) were HAdV-2 in subgroup C, 70 cases (7.4%) were HAdV-1, 16 cases(1.7%) were HAdV-5, and 6 cases(0.6%) were HAdV-4 in subgroup E. HAdV-14, HAdV-37, HAdV-40 and HAdV-41 were not detected.A total of 51 positive samples of HAdV infection detected by multiplex PCR were randomly selected to compare with the detection result by the first-generation sequencing, which were all consistent.Conclusions:This study successfully established a multiplex PCR based on capillary electrophoresis in diagnosing common serotypes of respiratory adenovirus infection in children.HAdV-3, HAdV-7 of subgroup B and HAdV-2 and HAdV-1 of subgroup C were the main serotypes of respiratory adenovirus infection in children of Wenzhou area.HAdV-14, HAdV-37, HAdV-40 and HAdV- 41 were not detected.
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Objective:To explore the clinical characteristics and risk factors of severe and critical influenza in children.Methods:The clinical data of 214 inpatient children with severe and critical influenza hospitalized in the Second Affiliated Hospital & Yuying Children′s Hospital of Wenzhou Medical University from January 1, 2016 to December 31, 2019 were retrospectively collected. The clinical characteristics including age, gender, symptoms, signs, underlying diseases, C-reactive protein (CRP), treatment and outcome of children with severe and critical influenza were compared. Chi-Square test was used for statistical analysis. A binary logistic regression model was constructed to analyze the risk factors for critically ill influenza.Results:Of the 214 children, 153 were male (71.5%), 177(82.7%) were under 5 years old. There were 52 children with underlying diseases. Fever occurred in 207 cases. Among the 54 cases that had convulsion during the course of the disease, three developed acute necrotizing encephalopathy. The influenza subtype was mainly type A, accounting for 190(88.79%). A total of 42(19.6%) children were critically ill. The incidence of critical influenza in children with underlying diseases (61.9%, 26/42) was higher than that in severe influenza children (15.1%, 26/172), and the difference was statistically significant ( χ2=40.175, P<0.01). The incidence of critical influenza in children with CRP≥40 mg/L was higher than that of severe influenza in children with CRP ≥40 mg/L (33.3%(14/42) vs 9.3%(16/172)), and the difference was statistically significant ( χ2=16.173, P<0.01). Multivariate logistic regression showed that underlying diseases (odds ratio ( OR)=8.794, 95% confidence interval ( CI) 3.845-20.111) and CRP ≥40 mg/L ( OR=5.050, 95% CI 1.966-12.970) were risk factors for critical influenza. All severe cases were improved and discharged.Among the 42 critically ill children, seven children died. Conclusions:Among the severe and critical influenza in children, the majority of children are under five years old.Underlying diseases and CRP ≥40 mg/L are risk factors for critical influenza.
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Protracted bacterial bronchitis (PBB) is one of the common causes of chronic cough in children.The common pathogenic bacteria of PBB include haemophilus influenzae, streptococcus pneumoniae and moraxella catabilis.Viruses are also involved in the development of PBB.In addition, the microbiome of PBB is different from that of normal children, with lower respiratory tract flora disorder.This paper summarized the recent progress in the microbiology of PBB.
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Plastic bronchitis(PB) is an uncommon respiratory disease characterized by formation of casts in tracheobronchial tree.It can lead to airway obstruction, difficulty of breathing and even respiratory failure.PB in children is commonly associated with lower airway infection, cyanotic congenital heart disease and asthma or atopic diseases.It can also be found in children with sickle cell anemia, thalassemia and cystic fibrosis and so no.There are three main mechanisms for the formation of casts: airway inflammation results in mucus hypersecretion; inflammatory insults lead to necrosis and abscission of the airway epithelium, mucosal edema, and finally cause airway clearance impairment; leakage of chyle from lung lymphatic circulation into airway.But the etiology of this disease is various, pathogenesis is complex.Further research is required to elucidate the pathogenesis.
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Bordetella pertussis is one of the most common respiratory infectious diseases leading to the death of infants in the world.With the introduction of pertussis vaccination and effective prevention and treatment measures, the incidence and mortality of pertussis decreased significantly.However, in the past 20 years, some countries, especially developed countries with relatively complete vaccination, have seen " re-emergence of pertussis" . At the same time, the incidence of pertussis in China is not optimistic, there is also a significant increase in the incidence of the phenomenon.This article reviews the epidemiology and clinical manifestations of Bordetella pertussis.
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Five patients with primary malignancies in the pterygopalatine fossa (PPF) and infra temporal fossa (ITF) were enrolled in this retrospective study between January 2012 and January 2018. After malignancies proven by biopsy and evaluation with CT and MRI scan, all patients received modified maxillary swing (MMS) approach for extirpation of malignant tumors in the PPF and ITF under general anesthesia. En bloc resection with wide surgical margins was successfully performed in all cases. Negative margins were observed in 4 cases and positive margins were found in one patient with adenoid cystic carcinoma who received postoperative radiotherapy. The most common complication was facial numbness. During the follow-up period (range 12 to 57 months), one patient suffered from recurrence while others did not. The advantages of MMS include wide surgical field, full exposure and easy manipulation. The MMS approach is expected to become an standard method for monobloc resection of malignancies in the PPF and ITF.
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Objective@#To understand the epidemiologic features of the rabies in Xishuang banna prefecture of Yunnan province, China in 2008-2017 and the viral molecular-evolution characteristics.@*Methods@#The data of rabies case questionnaire were collected. The brain tissue samples from mad dogs, suspicious sick dogs and human brain tissue, saliva and cerebrospinal fluid samples from rabies patients were collected in Xishuangbanna. Coding region of nucleoprotein and glycoprotein genes were amplified by RT-PCR and sequenced. Homology and phylogenetic analysis were performed using the relevant bioinformatics software.@*Results@#A total of 62 cases of human rabies were occurred in 28 districts of the 3 counties, Xishuangbanna prefecture in 2008-2017. Of them, 37 cases in Jinghong county, 15 in Menghai county and 10 in Mengla county. In which 48 cases were bitten by domestic dogs (77.42%), 11 cases were bitten by wild dogs (17.74%). Rabies case was occurred every year in the past decade. The seasonal incidence was not obvious. The majority of patients were aged from 30 to 59 years-old, with the youngest 1 year-old and the eldest 91 year-old. The male to female ratio was 1.70∶1, most cases were farmers. The nucleotide sequences of nucleoprotein gene of 9 virus strains (7 from Jinghong, 1 from Menghai and 1 from Mengla) were obtained from the samples of dogs and patients. Homology and phylogenetic analyses indicated that the 5 strains belonged to clade China-Ⅰ, 3 clade China-Ⅱ and 1 clade China-Ⅵ. The nucleotide sequences of glycoprotein gene of 5 virus strains (3 from Jinghong, 1 from Menghai and 1 from Mengla) were obtained from these positive samples, and all were clade China-Ⅰ, it is same with nucleoprotein genes analysis result from these 5 virus strains. These China-Ⅰ and China-Ⅱ strains from Xishuangbanna have a closer genetic relationship with same clade strains isolated from Pu’er and other prefectures of Yunnan province as well as Sichuan, Guizhou and Guangxi. The China-Ⅵ strain from Xishuangbanna share high homology and genetic relationship with China-Ⅵ strains isolated from southwestern Yunnan and neighbouring countries such as Myanmar, Laos and Vietnam in recent years.@*Conclusions@#In Xishuangbanna, rabies mainly occurred in rural area and domestic dog was the main source of transmission. These RABV clades China-Ⅰ, China-Ⅱ and China-Ⅵ were found in this region and the China-Ⅰ was principal clade. The transmission source of China-Ⅰ and China-Ⅱ were from adjacent areas in the province and China-Ⅵ was from Myanmar and Laos.
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There is still much work to be done in the diagnosis, treatment, prevention and control of tuberculosis among primary and middle schools and students.Medical institutions, especially the pediatricians, who can cooperate with Centers for Disease Control and education departments, should play a bigger role in propaganda of prevention knowledge in tuberculosis among primary and middle schools and students, tuberculosis screening, prophylactic treatment of latent tuberculosis infection, risk factors research and follow-up studies of tuberculosis.
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Objective To investigate the current situations of the frailty and the perceived social support and explore the influence of social support on the frailty in elderly maintenance hemodialysis patients.Methods The 237 elderly patients with maintenance hemodialysis were investigated by the Fried frailty phenotype and the perceived social support scale in this cross-sectional study.Results The prevalence of frailty was 163 (68.8％)in 237 patients.The level of perceived social support was low.The frailty was negatively correlated with the social supportscore,family support,friends support,and other support(r =-0.326,-0.129,-0.301 and-0.230,respectively,all P ＜0.05).Multiple logistic regression analysis showed that friends support,dialysis vintage,Charlson comorbidity index(CCI)score and activities of daily living(ADL)score were the main influential factors for frailty in elderly maintenance hemodialysis patients.Conclusions Medical staff should evaluate the frailty in elderly patients with maintenance hemodialysis,and the frailty can be alleviated by improving the support of friends.
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There is still much work to be done in the diagnosis,treatment,prevention and control of tuberculosis among primary and middle schools and students.Medical institutions,especially the pediatricians,who can co operate with Centers for Disease Control and education departments,should play a bigger role in propaganda of prevention knowledge in tuberculosis among primary and middle schools and students,tuberculosis screening,prophylactic treatment of latent tuberculosis infection,risk factors research and follow-up studies of tuberculosis.
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Objective@#To illustrate the epidemical characteristics of the dog-biting events and molecular evolution of rabies virus (RV) strains prevalent in Shuangbai county of Yunnan province, China.@*Methods@#Epidemical investigation on the dog-biting events and human cases were conducted and the brain tissues of the biting dogs and human cases were sampled post-mortem. Nucleoprotein (N) genes of the RVs were sequenced. Homology and phylogenetic analysis were performed using the relevant bioinformatics software.@*Results@#A total of 12 dog-biting events took place between 2011-2017 in Shuangbai county and 35 persons were bitten. Of the 12 events, 11 were investigated in time and 32 bitten persons received proper wound management and a full post-exposure vaccination course. Rabies has not developed in these wounded cases until now. However, due to failure to receive medical intervention and post-exposure treatment in time, 1 of 3 bitten persons in a single event died of rabies. RV N genes from 5 dogs and 1 person were sequenced. Phylogenetic tree showed that RV strains prevalent in Shuangbai county were closely related with the ones found in neighboring counties/cities such as Chuxiong, Lufeng, Jingdong and Xiangyun. All these strains were related to the ones denoted as clade China-I and prevalent in Sichuan province. Homology analysis showed 99.6%-100% homology in nucleotide and amino acid among the 6 RVs prevalent in Shuangbai county and those prevalent in Chuxiong, Lufeng, Xiangyun and clade China-I of Sichuan province. Compared with the China-I strains prevalent in Chuxiong, Zhaotong and Qujing prefectures between 2006-2007, the homology of nucleotide and amino acid were 97.1%-99.3% and 99.1%-99.6%, respectively.@*Conclusions@#Surveillance on the dog-biting events can prevent rabies in humans effectively. RV strains prevalent in Shuangbai county belong to clade China-I and have a close relationship with those of neighboring prefectures, cities, counties and the ones prevalent in Sichuan province.
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Objective To compare effect between thyroid imaging reporting and data system (TI-RADS) and the Bethesda system for reporting thyroid cytopathology (TBSRTC) in the qualitative diagnosis of thyroid nodule. Methods The clinical data of 665 patients with thyroid nodule (1 598 nodules) from April 2013 to October 2016 were retrospectively analyzed. TBSRTC and TI-RADS were used for qualitative diagnosis of thyroid nodule before operation. Pathological diagnosis was performed after the operation. The diagnostic effect of TI-RADS and TBSRTC were assessed, and the factors leading to the diagnostic errors were analyzed. Results Of 1 598 thyroid nodules, the pathological diagnosis showed that benign nodules were in 202, and malignant thyroid nodules were in 1 396. The diagnostic sensitivity and 85.64%(173/202) vs. 74.75%(151/202) and specificity of malignant nodules by TBSRTC were significantly higher than that by TI-RADS: 91.76% (1 281/1 396) vs. 87.11% (1 216/1 396), and there was statistical difference (P＜0.01). The diagnosis positive rate of malignant nodules with diameter＜1 cm by TBSRTC was significantly higher than that by TI-RADS: 77.63% (59/76) vs. 47.37% (36/76), and there was statistical difference (P＜0.01); there was no statistical difference in diagnosis positive rate of malignant nodules with diameter ≥ 1 cm between 2 methods (P＞0.05). Univariate analysis result showed that the diameter of thyroid nodules in patients with TI-RADS false negative was significantly smaller than that in patients with benign thyroid nodules: (1.01 ± 0.48) cm vs. (1.51 ± 0.45) cm, the incidence of malignant thyroid nodules combined with other thyroid diseases was significantly higher than that in patients with benign thyroid nodules: 41.18% (21/51) vs. 11.32% (158/1 396), and there were statistical differences (P＜0.05); the rate of thyroid dysfunction in patients with TBSRTC false positive was significantly higher than that in patients with malignant thyroid nodules: 18.26% (21/115) vs. 6.93% (14/202), and there was statistical difference (P＜0.05). Conclusions The qualitative diagnosis of thyroid nodule requires the cross-reference of TI-RADS and TBSRTC, and the combination of other clinical indicators of patients can improve the detection rate of malignant thyroid nodules.
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Objective To investigate the clinical,laboratory and imaging characteristics of children with Mycoplasma pneumoniae pneumonia (MPP) in different age groups.Methods One hundred and sixty-four children whose ages were 1 month to 14 years old with MPP hospitalized at Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine in 2017 were enrolled as study objects.All children were classified into 2 groups:1 month to 3 year-old group (46 cases) and ＞ 3 to 14 year-old group (118 cases).The clinical features,main laboratory and imaging findings of 2 groups were retrospectively analyzed and compared.Results (1) Compared with 1 month to 3 year-old group,＞ 3 to 14 year-old group had more patients with fever [97.5％ (115/118 cases) vs.84.8％ (39/46 cases)],longer duration of fever [48.3％ (57/118 cases) vs.23.9％ (11/46 cases)] and higher fever [83.1％ (98/ 118 cases) vs.52.2％ (24/46 cases)],but less patients with wheezing [2.5％ (3/118 cases) vs.43.5％ (20/46 cases)],shortness of breath and dyspnea [0.8％ (1/118 cases) vs.15.2％ (7/46 cases)],dry rale [1.7％ (2/118 cases) vs.60.9％ (28/46 cases)] and wet pulmonary rale [50.0％ (59/118 cases) vs.69.6％ (32/46 cases)],and the differences were statistically significant (all P ＜ 0.05).(2) The increase of platelet count,creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) in 1 month to 3 year-old group of MPP [316.0 (229.3,372.3) × 109/L,25.70 (17.70,31.98) U/L,346.5 (310.3,388.3) U/L] were more common than those in ＞ 3 to 14 year-old group [266.0 (205.8,317.8) × 109/L,21.50 (15.75,28.00) U/L,303.0 (173.0,352.0) U/L],while the increase of C reactive protein (CRP) and erythrocyte sedimentation rate (ESR) in ＞ 3 to 14 year-old group [12.66(9.16,19.44) mg/L,23.00(17.75,29.00) mm/1 h] were more common than those in 1 month to 3 year-old group[2.46 (0.54,11.63) mg/L,14.00 (10.00,20.25) mm/1 h],and there were significant statistical differences (all P ＜ 0.05).(3) The MPP imaging features of children in different age groups were diverse.In 1 month to 3 year-old group,the double lung involvement [80.4％ (37/46 cases)] was more obvious than that in ＞3 to 14 year-old group [55.9％ (66/118 cases)],and the difference was statistically significant (P ＜ 0.05).The lesions of 1 month to 3 year-old group were more common in lobular pulmonary [82.6％ (38/46 cases)],while in ＞ 3 to 14 year-old group,the radiographic findings were predominant with consolidation in the segment or lobe of the lung [33.1％ (39/118 cases)],and the difference was statistically significant (P ＜ 0.01).Conclusions Younger than 3 year-old children with MPP are prone to wheezing,shortness of breath,dyspnea and lung rale,while the rise of CK-MB and LDH is common.Imaging is mainly involved in double lung involvement and pulmonary lobule infiltration.The older the children with MPP are,the longer the fever duration is,and more common is the higher fever.The older the children,the more more easily they have the relative increase of CRP and ESR,and at the same time they are more likely to have the consolidation of the lung segment or lobe.
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Objective To evaluate effects of early rehabilitation nursing program on the patients with first-attack ischemic stroke.Methods One hundred and thirty-one patients with first-attack ischemic stroke were selected and divided into control group(65) and intervention group(66).The control group was given conventional neurology nursing and the intervention group was given intervention of early rehabilitation nursing program.Before and after intervention,the patients were assessed with the National Institutes of Health Stroke Scale(NIHSS) and Barthel Index(BI).After 3 months of intervention,the patients were assessed with simplified modified Rankin Scale questionnaire(smRSq).Results At the end of 2 weeks after intervention,NIHSS scores of the intervention group was lower than that of in the control group[(3.41±2.36) scores vs.(4.49± 3.65) scores,P＜0.05],and the Barthel index scores was higher than that of control group[(72.65t±15.69) scores vs.(56.92±21.99)scores,P＜0.05].After 3 months of intervention,the difference of smRSq(71.21％ vs.49.23％,P＜0.05) scores between them were significant(P＜0.05).Conclusion Early rehabilitation nursing program can significantly improve the neurological function and daily life ability of patients with initial ischemic stroke,reducing the disability rate and improve the quality of life.
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Objective:To set up the rat skeletal muscle L6 cell models of oxygen-glucose deprivation(OGD)in vitro,and to investigate the protective effect of EGF in deep tissue inj ury(DTI)of pressure sores.Methods:The rat skeletal muscle cells in the logarithmic phase were divided into normal control group,OGD group,5 μg·L-1 EGF+OGD group,10 μg·L-1EGF+ OGD group and 20 μg·L-1EGF+ OGD group.The survival rates of skeletal muscle cells in various groups were measured by MTT assay;the cell apoptotic rates in various groups were detected by flow cytometry;the reactive oxygen species(ROS)levels were detected by DCFH-DA;Rhodamine 123 was used to detect the mitochondrial membrane potential;the expressions of Bax and Bcl-2 proteins were determined by Western blotting method.Results:Compared with normal control group,the survival rates of skeletal muscle cells in OGD group after 24 h OGD was significantly decreased(P<0.05);the apoptotic rate was markedly increased(P<0.01);the ROS level was increased(P<0.01);the mitochondrial membrane potential was decreased(P<0.01);the ratio of Bcl-2/Bax was significantly decreased(P<0.01).Compared with OGD group, the survival rates of skeletal muscle cells in different concentrations of EGF groups were increased and the apoptotic rates were decreased,especially in 10 and 20 μg·L-1EGF groups(P<0.05 or P<0.01);the ROS levels in skeletal muscle cells in different concentrations of EGF groups were decreased and the mitochondrial membrane potential were increased,especially in 10 and 20 μg·L-1EGF groups(P<0.05 or P<0.01);the Bcl-2/Bax ratios were significantly decreased in a concentration-dependent manner,especially in 10 and 20 μg·L-1EGF groups (P<0.05 or P<0.01).Conclusion:EGF can improve the skeletal muscle cell injury induced by OGD in a concentration-dependent manner via decreasing the ROS levels and protecting the cell mitochondrial function.
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Objective To investigate the expression of human epidermal growth factor receptor 2 (HER2)and its relationship with prognosis in breast cancer patients.Methods One hundred and ninety-eight patients with breast cancer treated with breast surgery in Jizhong Energy Fengfeng Group Hospital from January 2007 to October 2011 were selected as the research objects.The expression of HER2 in 198 cases of breast cancer and 108 cases of breast adenoma at the same time were compared.The 5 year disease-free survival and overall survival rate of breast cancer patients with HER2 positive and negative expression were analyzed and compared.Cox regression analysis was used to analyze the prognosis.Results The positive expression of HER2 in breast cancer tissues was showed in 93 cases,the negative expression was observed in 105 cases,the positive rate was 47.0%(93/198); the positive rate in the breast adenoma group was 0(0/108).The difference between the two groups in the positive rate was statistically significant(P=0.000).The 5 year disease-free survival rate and overall survival rate of the breast cancer patients in the HER2 positive group was 80.8% and 82.9%,respectively.The 5 year disease-free survival rate and overall survival rate was 94.6% and 97.2% in the HER2 negative group,respectively.The differences between the two groups was statistically significant(P=0.046,0.045).The tumor diameter,clinical stage,lymph node metastasis and HER2 positive expression were the independent risk factors for disease-free survival rate and overall survival rate of breast cancer patients(RR=1.59,1.51,5.45,1.72,95%CI=1.00 ~ 2.50,1.10 ~ 17.03,2.00 ~ 5.39,1.10~1.68,P<0.05).The tumor diameter,clinical stage,lymph node metastasis and HER2 positive expression were the independent risk factors for overall survival rate of breast cancer patients(RR=1.70,1.630,4.29,2.08,95%CI=1.02~2.80, 1.12~15.87,2.40~7.59,1.28~3.42,P<0.05).Conclusion Overexpression of HER2 in breast cancer can promote the occurrence,development and metastasis of breast cancer and that is an independent risk factor to predict the prognosis of breast cancer patients.
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Objective To investigate the expression and clinical significance of Survivin and human epidermal growth factor receptor-2 in breast cancer tissue.Methods Eighty-seven cases of patients with breast cancer treated in Jizhong Energy Fengfeng Group Hospital from October 2010 to December 2016 were analyzed.All of them were diagnosed as invasive ductal carcinoma of breast by pathological diagnosis.Normal tissue adjacent to the tumor(more than 5 cm)was collected as the control group.The expression of Survivin and human epidermal growth factor receptor -2 in breast lesions was detected by immunohistochemical SP method.The correlation between Survivin and human epidermal growth factor receptor -2 was analyzed through the comparison of their expression of different clinical pathological features in breast cancer patients.Results The positive rate of Survivin in the breast cancer group was 69.0%(60/87),and 4.6%(4/87)in the control group.The difference between the two groups was statistically significant(P=0.000).The positive rate of human epidermal growth factor receptor -2 in cancer tissues was 46%(40/87)in the breast cancer group,and 0(0/87)in the control group.The difference between the two groups was statistically significant(P=0.000).The expression of survivin was not related to the age,tumor diameter and histological grade of breast cancer patients, but was related to lymph node metastasis(P<0.05).The expression of human epidermal growth factor receptor-2 was not related to the age,tumor diameter of breast cancer patients,but was related to tissue grading and lymph node metastasis(P<0.05).Spearman correlation analysis showed that human epidermal growth factor receptor-2 was positively correlated with survivin(r=0.412,P<0.05).Conclusion Survivin and human epidermal growth factor receptor-2 are associated with the development and progression of breast cancer,both of which play a coordinating role in the development and progression of breast cancer.They play an important role in the process of tumor invasion and metastasis.
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Objective To investigate the expression and clinical significance of Survivin and human epidermal growth factor receptor-2 in breast cancer tissue.Methods Eighty-seven cases of patients with breast cancer treated in Jizhong Energy Fengfeng Group Hospital from October 2010 to December 2016 were analyzed.All of them were diagnosed as invasive ductal carcinoma of breast by pathological diagnosis.Normal tissue adjacent to the tumor(more than 5 cm)was collected as the control group.The expression of Survivin and human epidermal growth factor receptor -2 in breast lesions was detected by immunohistochemical SP method.The correlation between Survivin and human epidermal growth factor receptor -2 was analyzed through the comparison of their expression of different clinical pathological features in breast cancer patients.Results The positive rate of Survivin in the breast cancer group was 69.0%(60/87),and 4.6%(4/87)in the control group.The difference between the two groups was statistically significant(P=0.000).The positive rate of human epidermal growth factor receptor -2 in cancer tissues was 46%(40/87)in the breast cancer group,and 0(0/87)in the control group.The difference between the two groups was statistically significant(P=0.000).The expression of survivin was not related to the age,tumor diameter and histological grade of breast cancer patients, but was related to lymph node metastasis(P<0.05).The expression of human epidermal growth factor receptor-2 was not related to the age,tumor diameter of breast cancer patients,but was related to tissue grading and lymph node metastasis(P<0.05).Spearman correlation analysis showed that human epidermal growth factor receptor-2 was positively correlated with survivin(r=0.412,P<0.05).Conclusion Survivin and human epidermal growth factor receptor-2 are associated with the development and progression of breast cancer,both of which play a coordinating role in the development and progression of breast cancer.They play an important role in the process of tumor invasion and metastasis.